macOS 27 developer beta 5 introduces a bug where an NSTrackingSeparatorToolbarItem in a toolbar causes a bar to appear under the toolbar, overlaying the content.
I have a blog post about this at: https://www.virtualsanity.com/202608/nstrackingseparatortoolbaritem-causes-white-bar-over-top-of-content-in-macos-27-developer-beta-5/
I reported this as FB24266969. A sample project and a screenshot are included in both the blog post and the feedback report.